Mayo Test ID SRSV Respiratory Syncytial Virus (RSV) Antibodies, IgG and IgM (Separate Determinations), Serum


Specimen Required


Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Useful For

Aiding in the diagnosis of a recent respiratory syncytial virus infection

 

Not useful for diagnosis from spinal fluid

Method Name

Immunofluorescence

Reporting Name

Respiratory Syncytial Virus, IgG/M

Specimen Type

Serum

Specimen Minimum Volume

0.15 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

NA

Other

NA

Clinical Information

Respiratory syncytial virus (RSV) is an important cause of human respiratory infection. It strikes most frequently and severely in the very young and is a common cause of bronchiolitis, pneumonia, or croup in young infants. Infections in older children and adults tend to be milder and to involve the upper respiratory tract. RSV infections are seasonal, from late fall to spring, and often occur in epidemic form.

Reference Values

IgG: <1:10

IgM: <1:10

Reference values apply to all ages.

Interpretation

The presence of IgM class antibodies or a 4-fold or greater rise in paired sera IgG titer indicates recent infection.

 

The presence of demonstrable IgG generally indicates past exposure and immunity.

Cautions

For low IgG antibody levels with no demonstrable IgM, it is recommended that a convalescent specimen be drawn in 2 to 4 weeks.

Clinical Reference

Tristram DA, Welliver RC: Respiratory syncytial virus. In Manual of Clinical Microbiology. Seventh edition. Edited by PR Murray, EJ Baron, MA Pfaller, et al. Washington, DC, ASM Press, 1999, pp 942-950

Method Description

The indirect immunofluorescence test is used for the measurement of IgG and IgM antibodies to respiratory syncytial virus (RSV). Specific antibodies present in the serum of the patient react with RSV-infected cells that have been previously fixed on a glass microscope slide. Fluorescein-labeled antihuman IgG or IgM conjugate is used to stain specific antibody bound to the substrate cells. The slides are examined with a fluorescence microscope for the characteristic apple-green fluorescence of the infected cells.(Urquhardt GE, Walker GH: Immunofluorescence for routine diagnosis of respiratory syncytial virus infection. J Clin Pathol 1972;25:843-845)

Day(s) and Time(s) Performed

Tuesday, Friday; 9 a.m.

Analytic Time

Same day/1 day

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86756 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SRSV Respiratory Syncytial Virus, IgG/M In Process

 

Result ID Test Result Name Result LOINC Value
5737 Respiratory Syncytial Virus, IgG 9573-7
5738 Respiratory Syncytial Virus, IgM 9574-5