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Mayo Clinic Laboratories

Mayo Test ID ABTIR Antibody Titer, Blood and Serum


Shipping Instructions


Specimen must arrive within 72 hours of collection.



Specimen Required


Blood cells, plasma, and serum are required.

Supplies: Sarstedt Aliquot Tube, 5mL (T914)

Specimen Type: Plasma/Blood

Collection Container/Tube: 6-mL Pink top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume:

3 mL Plasma

3 mL Red blood cells (RBCs)

Collection Instructions:

1. Centrifuge and aliquot plasma into plastic vial.

2. Label specimens as EDTA plasma or EDTA RBCs as appropriate.

3. Send both tubes.

 

Specimen Type: Serum/Blood

Collection Container/Tube: 10-mL Red top

Submission Container/Tube: Plastic vial

Specimen Volume:

5 mL Serum

5 mL RBCs

Collection Instructions:

1. Centrifuge and aliquot serum into a plastic vial.

2. Label specimens as serum or clotted RBCs as appropriate.

3. Send both tubes.


Useful For

Monitoring antibody levels during pregnancy to help assess the risk of hemolytic disease of the newborn

 

This test is not useful for monitoring the efficacy of Rh-immune globulin administration.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
ABIDR Antibody Identification, RBC Yes No

Testing Algorithm

If the antibodies detected are too weakly reactive to titer, this test will be canceled and the antibody identification will be ordered and performed at an additional charge.

Method Name

Hemagglutination

Reporting Name

Antibody Titer, RBC

Specimen Type

Varies

Specimen Minimum Volume

Blood: 6 mL EDTA
Pediatric: 2 mL Serum

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred) 4 days
  Refrigerated  4 days

Reject Due To

No specimen should be rejected.

Clinical Information

Some maternal IgG alloantibodies to red blood cell antigens will cross the placenta and cause hemolysis of antigen-positive fetal red blood cells. The resulting fetal anemia and hyperbilirubinemia can be harmful or possibly fatal to the newborn.

Reference Values

Negative

If positive, result will be reported as the reciprocal of the highest dilution at with macroscopic agglutination (1+) is observed.

Interpretation

The specificity of the maternal alloantibody will be stated. The titer result is the reciprocal of the highest dilution at which macroscopic agglutination (1+) is observed.

 

If the antibody problem identified is not relevant in hemolytic disease of the newborn or if titrations are not helpful, the titer will be canceled and will be replaced by ABIDR / Antibody Identification, Blood and Serum. 

 

A consultation service is offered, at no charge, regarding the clinical relevance of red blood cell antibodies.

Cautions

Recent administration of Rh-immune globulin may cause anti-D to be identified and appear falsely as an alloantibody.

Clinical Reference

Cohn CS, Delaney M, Johnson ST, Katz LM, Schwartz J. eds: Technical Manual. 21st ed. AABB; 2023

Method Description

The strength and specificity of the antibody to be titered is first determined. Two-fold serial dilutions of serum are tested against antigen-positive erythrocytes. The result is the reciprocal of the highest dilution at which macroscopic agglutination (1+) is observed at the antihuman globulin phase of testing. Parallel titration of a previous specimen of the patient's serum (frozen) provides a baseline for comparison of antibody level. In the absence of a previous specimen from the patient, parallel titration of a control antiserum is used for standardization.(Cohn CS, Delaney M, Johnson ST, Katz LM, Schwartz J. eds: Technical Manual. 21st ed. AABB; 2023)

Day(s) Performed

Monday through Friday, Sunday

Report Available

1 to 2 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86886

86870 (if appropriate-per panel tested)

86860 (if appropriate)

86880 x 3 (if appropriate)

86905- (if appropriate)

86978 (if appropriate)

81403 (if appropriate)-Internal only

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ABTIR Antibody Titer, RBC 50962-0

 

Result ID Test Result Name Result LOINC Value
SPECR Antibody Specificity 888-8
ALTIR Allo Antibody Titer In Process
CTPSR Current Titer of Previous Specimen In Process