Home
HelpMayo Test ID BARBU Barbiturates Confirmation, Random, Urine
Ordering Guidance
For situations where chain of custody is required, a Chain-of-Custody Kit (T282) is available. For chain-of-custody testing, order BARBX / Barbiturates Confirmation, Chain of Custody, Random, Urine.
Additional drug panels and specific requests are available. Call 800-533-1710 or 507-266-5700.
Additional Testing Requirements
If urine creatinine is required or adulteration of the sample is suspected, also order ADULT / Adulterants Survey, Random, Urine in addition to this test.
Specimen Required
Supplies: Urine Tubes, 10 mL (T068)
Container/Tube: Plastic, 10-mL urine tube
Specimen Volume: 10 mL
Collection Instructions:
1. Collect a random urine specimen.
2. No preservative.
Additional Information:
1. No specimen substitutions.
2. STAT requests are not accepted for this test.
Useful For
Detecting drug abuse involving barbiturates such as amobarbital, butalbital, pentobarbital, phenobarbital, and secobarbital
Method Name
Gas Chromatography Mass Spectrometry (GC-MS)
Reporting Name
Barbiturates Confirmation, USpecimen Type
UrineSpecimen Minimum Volume
5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Urine | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days | ||
| Ambient | 72 hours |
Reject Due To
| Gross hemolysis | OK |
| Gross icterus | OK |
Clinical Information
Barbiturates represent a class of drugs that were originally introduced as sleep inducers. Butalbital is also used to control severe headaches. Mephobarbital and phenobarbital are frequently used to control major motor (grand mal) seizures. These drugs are commonly abused as "downers" to induce sleep after an amphetamine- or cocaine-induced "high."
Reference Values
Negative
Cutoff concentrations:
BUTALBITAL BY GC-MS
<100 ng/mL
AMOBARBITAL BY GC-MS
<100 ng/mL
PENTOBARBITAL BY GC-MS
<100 ng/mL
SECOBARBITAL BY GC-MS
<100 ng/mL
PHENOBARBITAL BY GC-MS
<100 ng/mL
Interpretation
The presence of a barbiturate in urine indicates use of one of these drugs.
Most of the barbiturates are fast acting; their presence indicates use within the past 3 days.
Phenobarbital, commonly used to control epilepsy, has a very long half-life. The presence of phenobarbital in urine indicates that the patient has used the drug sometime within the past 30 days.
Cautions
No significant cautionary statements.
Clinical Reference
1. Baselt RC. Disposition of Toxic Drugs and Chemicals in Man. 10th ed. Biomedical Publications; 2014
2. Langman LJ, Bechtel LK, Holstege CP. Clinical toxicology. In: Rifai N, Chiu RWK, Young I, Burnham CAD, Wittwer CT, eds. Tietz Textbook of Laboratory Medicine. 7th ed. Elsevier; 2023:chap 43
Method Description
Gas chromatography mass spectrometry confirmation with quantitation.(Unpublished Mayo method)
Day(s) Performed
Monday, Wednesday, Friday
Report Available
3 to 5 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
80345
G0480 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BARBU | Barbiturates Confirmation, U | 53746-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 21442 | Butalbital-by GC/MS | 16237-0 |
| 20642 | Amobarbital-by GC/MS | 16239-6 |
| 20643 | Pentobarbital-by GC/MS | 16240-4 |
| 20644 | Secobarbital-by GC/MS | 16238-8 |
| 20645 | Phenobarbital-by GC/MS | 16241-2 |
| 20651 | Barbiturates Interpretation | 69050-3 |
| 20647 | Chain of Custody | 77202-0 |