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HelpMayo Test ID EOSU1 Eosinophils, Random, Urine
Specimen Required
Container/Tube: Unstained cytospin slide
Specimen Volume: 1 slide
Collection Instructions:
1. Collect a random urine specimen.
2. Centrifuge 10 mL of a random urine collection.
3. Pour off supernatant. Assess urine sediment for presence of white blood cells (WBC). If no WBC are seen, the eosinophil test cannot be performed. If WBC are present, prepare slides using the centrifuged sediment.
4. The unstained slide should be prepared within 2 hours of collection. Only an unstained slide will be accepted. Any other specimen, including the raw urine specimen, will be rejected.
5. Centrifuge urine in cytospin centrifuge at speed of 750 rpm for 5 minutes.
6. Slides need to be labeled with patient name and date of birth
7. Guidelines for slide preparation. Use the sediment to prepare slides as follows:
a. 150 mcL of sample for 1 to 3 WBC/high power field (hpf)
b. 100 mcL of sample for moderate to normal sediment (<50 cell/hpf)
c. 50 mcL of sample for heavier sediment (>50 cells/hpf)
d. 25 mcL for packed fields of sediment
Useful For
Investigation of possible acute interstitial nephritis
Method Name
Hansel Stain
Reporting Name
Eosinophils, USpecimen Type
UrineSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Urine | Ambient (preferred) | 14 days | SLIDE |
| Frozen | 14 days | SLIDE | |
| Refrigerated | 14 days | SLIDE |
Reject Due To
| Stained slides Urine aliquots |
Reject |
Clinical Information
Eosinophils are white blood cells that normally do not appear in urine. The presence of eosinophils in the urine is seen in acute interstitial nephritis, which is caused by an allergic reaction, typically to drugs.
Reference Values
0%
Interpretation
Results of greater than 5% eosinophils are indicative of acute interstitial nephritis; results between 1% and 5% eosinophils are indeterminant.
Cautions
No significant cautionary statements
Clinical Reference
1. Hansel FK. In: Clinical Allergy. CV Mosby Company; 1953
2. Brunzel NA. Microscopic examination of urine sediment. In: Fundamentals of Urine and Body Fluid Analysis. 4th ed. Saunders; 2017:141
3. Muriithi AK, Leung N, Valeri AM, et al. Biopsy-proven acute interstitial nephritis, 1993-2011: a case series. Am J Kidney Dis. 2014;64(4):558-566. doi:10.1053/j.ajkd.2014.04.027
Method Description
Hansel stain.(Brunzel NA, ed: Microscopic examination of urine sediment. In: Fundamentals of Urine and Body Fluids Analysis. 3rd ed. Elsevier; 2012:171-172)
Specimen Retention Time
Urine: 2 days; Slide: 7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
85999
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| EOSU1 | Eosinophils, U | 32014-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| EOSU1 | Eosinophils, U | 32014-3 |
Day(s) Performed
Monday through Sunday