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HelpMayo Test ID EXHR Hematologic Disorders, DNA and RNA Extract and Hold, Varies
Shipping Instructions
Refrigerate specimens must arrive within 5 days of collection, and ambient specimens must arrive with 3 days (72 hours) of collection. Collect and package specimens as close to shipping time as possible.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Blood
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD)
Specimen Volume: 10 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
3. Label specimen as blood.
Specimen Type: Bone marrow
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD)
Specimen Volume: 4 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Send bone marrow specimen in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Useful For
Reserving nucleic acids on any specimen for which molecular analysis requiring DNA or RNA may be necessary at a future date, ensuring that adequate material for testing is available
Testing Algorithm
DNA and RNA will be extracted from the specimen and stored at -80° Celsius for one year from the date the extraction process was performed. A report of "Performed" will be sent and a $75.00 processing fee will be assessed. To request molecular testing from the Molecular Hematopathology Laboratory's test menu, call 800-533-1710. Additional charges will be assessed for testing performed.
For more information see Eosinophilia: Bone Marrow Diagnostic Algorithm.
Special Instructions
Method Name
Nucleic Acid Extraction
Reporting Name
DNA and RNA Extract and HoldSpecimen Type
VariesSpecimen Minimum Volume
Blood: 8 mL
Bone Marrow: 2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 5 days | PURPLE OR PINK TOP/EDTA |
| Ambient | 72 hours | PURPLE OR PINK TOP/EDTA |
Reject Due To
| Gross hemolysis | Reject |
| Paraffin-embedded material Fluids Heparinized samples |
Reject |
Clinical Information
It is frequently useful to obtain nucleic acids from clinical samples containing a hematopoietic neoplasm at the time of diagnosis so appropriate material is available for molecular analysis should subsequent testing be required. For example, when a diagnosis of acute myelogenous leukemia is made, there is a delay before karyotype information, which determines whether testing for molecular prognostic markers is necessary, is available. After this delay, the diagnostic sample is usually no longer available or the nucleic acid has degraded to such an extent that it is no longer adequate for testing. Thus, it is useful to obtain nucleic acids on such specimens promptly at diagnosis and retain it until it is known whether additional testing is necessary.
Reference Values
Not applicable
Interpretation
A report of "Performed" will be sent and a $75 processing fee will be assessed. No interpretation will be given. Should the sample be used in future testing, interpretation would be incorporated with the final testing.
Cautions
DNA/RNA will be held at -80° Celsius for one year from the time of extraction. The nucleic acids may only be used for suitable tests offered by the Molecular Hematopathology Laboratory at Mayo Clinic. Nucleic acid samples cannot be shipped to another institution for testing and cannot be released for research use.
Only liquid blood specimens will be processed. Paraffin-embedded material and fresh, solid tissue will not be accepted at this time.
Method Description
DNA and RNA are extracted using automated systems. Both systems include a sample lysis step followed by binding of nucleic acid to a solid phase for separation from other cellular components and a final nucleic acid elution step.
Day(s) Performed
Monday through Saturday
Report Available
5 daysSpecimen Retention Time
Blood/Bone marrow: 2 weeks; Extracted DNA and RNA: 1 yearPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
Not ApplicableLOINC Code Information
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MP035 | Specimen Type | 31208-2 |
| 41938 | DNA/RNA Extract and Hold Result | No LOINC Needed |
| 41937 | DNA/RNA Extraction | No LOINC Needed |