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HelpMayo Test ID FDERM Fungal Culture, Dermal
Shipping Instructions
Specimen must arrive within 7 days of collection.
Transport in petri dishes may result in loss of specimen. Securely tape petri dishes closed for transport.
Necessary Information
Specimen source is required.
Specimen Required
Note:
-Aseptic techniques should be used when collecting specimens to minimize contamination.
-For optimal recovery of organisms, sufficient clinical material should be collected.
Specimen Type: Hair
Container/Tube: Dry sterile container or specimen collection envelope
Specimen Volume: 10 to 12
Collection Instructions: Using forceps collect affected hairs with base of the shaft intact.
Specimen Type: Nails
Container/Tube: Dry sterile container or specimen collection envelope
Specimen Volume: Entire collection
Collection Instructions:
1. Wipe the nail with 70% alcohol using gauze (not cotton).
2. Clip away a generous portion of the affected area.
3. Collect material or debris from under the nail.
Specimen Type: Skin
Container/Tube: Dry sterile container or specimen collection envelope
Specimen Volume: Entire specimen
Collection Instructions:
1. Cleanse the affected area with 70% alcohol.
2. Gently scrape the surface of the skin at the active margin of the lesion, being careful to not draw blood.
Useful For
Recovery and identification of dermatophyte fungi from hair, skin, and nail infected specimens
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| FUNA | Fungal Ident Panel A | No, (Bill Only) | No |
| FUNB | Fungal Ident Panel B | No, (Bill Only) | No |
| LCCI | Ident Rapid PCR Coccidioides | No, (Bill Only) | No |
| LCHB | Id, Histoplasma/Blastomyces PCR | No, (Bill Only) | No |
| RMALF | Id MALDI-TOF Mass Spec Fungi | No, (Bill Only) | No |
| RMALY | Id MALDI-TOF Mass Spec Yeast | No, (Bill Only) | No |
| D2F | D2 Fungal Sequencing Identification | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, the reflex test may be performed at an additional charge.
Method Name
Plated to Mycobiotic Agar
Reporting Name
Fungal Culture, DermalSpecimen Type
VariesSpecimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient | 7 days |
Reject Due To
| Charcoal, wooden shaft, or dry swab Agar plate Blades from scalpel or razor |
Reject |
Clinical Information
Fungal infections of keratinized tissues (hair, skin, nails) can be caused by dermatophytic fungi belonging to the genera Epidermophyton, Microsporum, and Trichophyton. Opportunistic superficial infections resembling dermatophytoses may be caused by yeasts or by unrelated filamentous fungi that are normally saprobes or plant pathogens. Dermatophytes are usually unable to penetrate deeper tissues. Infection may range from mild to severe.
Reference Values
Negative
If positive, fungus or yeast will be identified.
Interpretation
Positive cultures are reported with organism identification.
Negative reports are issued after 30 days incubation.
Cautions
No significant cautionary statements
Clinical Reference
Borman AM, Summerbell RC: Trichophyton, Microsporum, Epidermophyton and agents of superficial mycoses. In: Carroll KC, Pfaller MA, Landry ML, et al. Manual of Clinical Microbiology. 12th ed. ASM Press; 2019:2208-2233
Method Description
Specimens are plated on mycobiotic agar, which contains chloramphenicol and cyclohexamide to inhibit bacterial and saprobic fungal contamination. Cultures are incubated at 30° C for 30 days. Identification of dermatophyte species is based on colony and microscopic morphology and polymerase chain-reaction (PCR), DNA sequencing or matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, when applicable.(Hall L, Wohlfiel S, Roberts GD: Experience with the MicroSeq D2 large-subunit ribosomal DNA sequencing kit for identification of filamentous fungi encountered in the clinical laboratory. J Clin Microbiol. 2004 Feb;42[2]:622-626; Theel ES, Hall L, Mandrekar J, Wengenack NL: Dermatophyte identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2011 Dec;49[12]:4067-4071)
Day(s) Performed
Monday through Sunday
Report Available
30 to 35 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87101-Fungal culture, dermal
87106-Id MALDI-TOF Mass Spec Yeast (if appropriate)
87107-Id MALDI-TOF Mass Spec Fungi (if appropriate)
87107-Fungal identification panel A (if appropriate)
87107-Fungal identification panel B (if appropriate)
87150-Identification rapid PCR Coccidioides (if appropriate)
87150 x 2-Identification Histoplasma/Blastomyces, PCR (if appropriate)
87153-D2 fungal sequencing identification (if appropriate)
87150-Id, Candida auris Rapid PCR (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| FDERM | Fungal Culture, Dermal | 580-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| FDERM | Fungal Culture, Dermal | In Process |