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HelpMayo Test ID HEMMF Hematologic Specified FISH, Varies
Ordering Guidance
Consult with the laboratory before ordering this test.
The fluorescence in situ hybridization (FISH) probes to be analyzed must be specified on the request when ordering, otherwise test processing may be delayed in order to determine the intended analysis. If specific probes are not provided, this test may be canceled by the laboratory.
If testing paraffin-embedded tissue, bone marrow, or blood specimen for specific nonhematologic malignancies is desired, order MISCF / Miscellaneous Studies Using Chromosome-Specific Probes, FISH. If this test is ordered in this situation, it will be canceled and MISCF ordered and performed as the appropriate test.
Necessary Information
1. A list of probes requested for analysis is required.
2. A reason for testing should be submitted with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed. If this information is not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.
3. A pathology and/or flow cytometry report may be requested by the laboratory to optimize testing and aid in interpretation of results.
Specimen Required
Submit only 1 of the following specimens:
Preferred
Specimen Type: Bone marrow
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 2-3 mL
Collection Instructions:
1. It is preferable to send the first aspirate from the bone marrow collection.
2. Invert several times to mix bone marrow.
3. Send bone marrow specimen in original tube. Do not aliquot.
Acceptable
Specimen Type: Whole Blood
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 6 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
Useful For
The detection of specific chromosomal abnormalities in hematologic malignancies
Testing Algorithm
This test includes a charge for the probe application, analysis, and professional interpretation of results for one probe set (2 individual fluorescence in situ hybridization probes). Additional charges will be incurred for all additional probe sets performed.
Appropriate ancillary probes may be performed at consultant discretion to render comprehensive assessment. Any additional probes will have the results included within the final report and will be performed at an additional charge.
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
Hematologic Specified FISHSpecimen Type
VariesSpecimen Minimum Volume
Blood: 2 mL
Bone marrow: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Clinical Information
Fluorescence in situ hybridization using gene-specific probes and various probe strategies can help characterize chromosome abnormalities in hematologic malignancies for diagnostic, prognostic, and therapeutic purposes.
Reference Values
An interpretive report will be provided.Interpretation
A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe.
The absence of an abnormal clone does not rule out the presence of a neoplastic disorder.
Cautions
This test is not approved by the US Food and Drug Administration, and it is best used as an adjunct to existing clinical and pathologic information.
Bone marrow is the preferred specimen type for this fluorescence in situ hybridization test. If bone marrow is not available, a blood specimen may be used if there are malignant cells in the blood specimen (as verified by a hematopathologist).
Clinical Reference
Swerdlow SH, Campo E, Harris NL, et al, eds.: WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. IARC Press; 2017
Method Description
This test is performed using commercially available and laboratory-developed probes. For enumeration and break-apart strategy probe sets, 100 interphase nuclei are scored; 200 interphase nuclei are scored when dual-color, dual-fusion fluorescence in situ hybridization probes are used. All results are expressed as the percent abnormal nuclei.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Report Available
9 to 12 daysSpecimen Retention Time
4 weeksPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88271 x 2, 88275, 88291-FISH Probe, Analysis, Interpretation; 1 probe set
88271 x 2, 88275-FISH Probe, Analysis; each additional probe set (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HEMMF | Hematologic Specified FISH | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 614267 | Result Summary | 50397-9 |
| 614268 | Interpretation | 69965-2 |
| 614269 | Result Table | 93356-4 |
| 614270 | Result | 62356-1 |
| GC117 | Reason for Referral | 42349-1 |
| GC118 | Probes Requested | 78040-3 |
| GC119 | Specimen | 31208-2 |
| 614271 | Source | 31208-2 |
| 614272 | Method | 85069-3 |
| 614273 | Additional Information | 48767-8 |
| 614274 | Disclaimer | 62364-5 |
| 614275 | Released By | 18771-6 |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| HEMMB | Probe, Each Additional (HEMMF) | No, (Bill Only) | No |