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HelpMayo Test ID HG34W Histone 3.3 G34W (H3F3A G34W) Immunostain, Technical Component Only
Ordering Guidance
Mayo Clinic Laboratories has multiple histone immunostains available. See table for ordering guidance.
|
Test ID |
Published name |
Indication |
Mayo Clinic |
|
HG34W |
Histone 3.3 G34W (H3F3A G34W) Immunostain, Technical Component Only |
Giant cell tumor of bone (GCTB) |
H3 G34W |
|
HK27M |
Histone H3 K27M Mutant (H3 K27M) Immunostain, Technical Component Only |
K27M mutant midline gliomas |
H3 K27M |
|
HISME |
Histone H3 Trimethyl K27 Immunostain, Technical Component Only |
MPNST and K27M mutant midline gliomas |
H3 K27me3 |
|
HK36M |
Histone H3 K36M Mutant (H3F3 K36M) Immunostain, Technical Component Only |
Chondroblastoma |
H3 K36M |
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Specimen Required
Supplies: Immunostain Technical Only Envelope (T693)
Specimen Type: Tissue
Container/Tube: Immunostain Technical Only Envelope
Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Acceptable: Formalin-fixed, paraffin-embedded tissue block
Useful For
Aiding in the diagnosis of giant cell tumor of bone
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| IHTOI | IHC Initial, Tech Only | No | No |
| IHTOA | IHC Additional, Tech Only | No | No |
Method Name
Immunohistochemistry (IHC)
Reporting Name
Histone H3 G34W IHC, Tech OnlySpecimen Type
TECHONLYSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| TECHONLY | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
| Wet/frozen tissue Cytology smears Nonformalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides |
Reject |
Clinical Information
H3F3A is a member of the histone H3 family and is located on chromosome 1. Histone H3 G34W is expressed in the nuclei of the mononuclear cell population of H3F3A altered giant cell tumor of bone (GCTB).
Interpretation
This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.
The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Cautions
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks.
Clinical Reference
1. Cleven A, Hocker S, Briaire-de Bruijn I, Szuhai K, Cleton-Jansen AM, Bovee JVMG. Mutation analysis of H3F3A and H3F3B as a diagnostic tool for giant cell tumor of bone and chondroblastoma. Am J Surg Pathol. 2015;39(11):1576-1583
2. Fernanda A, Bersha F, Ye H, et al. H3F3A (Histone 3.3) G34W immunohistochemistry a reliable marker defining benign and malignant giant cell tumor of one. Am J Surg Pathol. 2017;41(8):1059-1068
3. Luke J, Von Baer A, Schreiber J, et al. H3F3A mutation in giant cell tumour of the bone is detected by immunohistochemistry using a monoclonal antibody against the G34W mutated site of histone H3/3 variant. Histopathology. 2017;71(4):125-133
4. Yamamoto H, Iwasaki T, Yuichi Y, et al. Diagnostic utility of histone H3.3 G34W, G34R, and G34V mutant-specific antibodies for giant cell tumors of bone. Hum Pathol. 2018;73(3):41-50
5. Magaki S, Hojat SA, Wei B, So A, Yong WH. An introduction to the performance of immunohistochemistry. Methods Mol Biol. 2019;1897:289-298. doi:10.1007/978-1-4939-8935-5_25
Method Description
Immunohistochemistry on sections of paraffin-embedded tissue.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysSpecimen Retention Time
Until staining is completePerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
88342-TC, Primary
88341-TC, If additional IHC
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HG34W | Histone H3 G34W IHC, Tech Only | Order only;no result |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 604698 | Histone H3 G34W IHC, Tech Only | Bill only; no result |
Testing Algorithm
For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).