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HelpMayo Test ID HOLDF Hematologic Disorders, Fluorescence In Situ Hybridization (FISH) Hold, Varies
Ordering Guidance
This test is designed to hold blood and bone marrow specimens only.
This test does not apply to chromosome analysis. If specimen is to be held for chromosome analysis, order HOLDC / Hematologic Disorders, Chromosome Hold, Varies.
Due to stability issues, test PCPDS / Plasma Cell Proliferative Disorder, High-Risk with Reflex Probes, Diagnostic FISH Evaluation, Bone Marrow cannot be added onto specimens held under this test.
Shipping Instructions
Advise Express Mail or equivalent if not on courier service.
Necessary Information
Provide a reason for testing with each specimen and bone marrow pathology report (if available). The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
Specimen Required
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Bone marrow
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (sodium heparin), lavender top (EDTA)
Specimen Volume: 1 to 2 mL
Collection Instructions: Invert several times to mix bone marrow.
Acceptable:
Specimen Type: Blood
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (sodium heparin), lavender top (EDTA)
Specimen Volume: 6 mL
Collection Instructions: Invert several times to mix blood.
Useful For
Processing the bone marrow or peripheral blood specimen but delaying fluorescence in situ hybridization analysis while preliminary morphologic assessment is in process
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| AMLAF | Adult AML, FISH | Yes | No |
| AMLMF | AML, Specified FISH | Yes | No |
| AMLPF | Pediatric AML, FISH | Yes | No |
| BALAF | Adult ALL (B-cell), FISH | Yes | No |
| BALMF | ALL (B-cell), Specified FISH | Yes | No |
| BALPF | Pediatric ALL (B-cell), FISH | Yes | No |
| BLPMF | B-cell Lymphoma, Specified FISH | Yes | No |
| CLLDF | CLL, Diagnostic FISH | Yes | No |
| CLLMF | CLL, Specified FISH | Yes | No |
| COGBF | COG, ALL (B-cell), FISH | Yes | No |
| COGMF | COG, AML, FISH | Yes | No |
| COGTF | COG, ALL (T-cell), FISH | Yes | No |
| EOSDF | Chronic Eosinophilia, Diag FISH | Yes | No |
| EOSMF | Chronic Eosinophilia, Spec FISH | Yes | No |
| HEMMF | Hematologic Specified FISH | Yes | No |
| MDSDF | MDS, Diagnostic FISH | Yes | No |
| MDSMF | MDS, Specified FISH | Yes | No |
| MFCDF | Myeloma Fixed Cell, High Risk, FISH | Yes | No |
| PHLDF | Ph-like ALL(B-cell), Diag FISH | Yes | No |
| TALAF | Adult ALL (T-cell), FISH | Yes | No |
| TALMF | ALL (T-cell), Specified FISH | Yes | No |
| TALPF | Pediatric ALL (T-cell), FISH | Yes | No |
| TLPDF | T-cell Lymphoma B/BM, Diag FISH | Yes | No |
| TLPMF | T-cell Lymphoma B/BM, Spec FISH | Yes | No |
Testing Algorithm
This test is designed to hold the sample and delay fluorescence in situ hybridization (FISH) testing while preliminary morphologic assessment or flow cytometry testing is in process.
Hold policy: Upon sample receipt, the specimen will be held in the laboratory. FISH testing will not be performed unless the client contacts the laboratory and indicates that FISH testing is desired. The client must contact the Cytogenetics Laboratory at 800-533-1710 by 4 p.m. (Central time) no later than 4 business days (96 hours) after the specimen was collected. If no notification is received by this time, the order will be processed as "canceled." Weekend communication can be deferred until Monday.
Method Name
Direct Preparation of Specimen
Reporting Name
Heme FISH Hold, B/BMSpecimen Type
VariesSpecimen Minimum Volume
Blood: 2 mL
Bone marrow: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Standard | |||
Reject Due To
No specimen should be rejected.Clinical Information
Fluorescence in situ hybridization (FISH) analysis using gene-specific probes is a useful methodology to detect common, recurrent chromosome abnormalities for most hematologic malignancies. Based on morphologic review of the bone marrow or peripheral blood specimen by a hematopathologist, a determination of additional appropriate testing can be made. If the specimen does not show evidence of malignancy, FISH analysis may not be necessary. Depending on the diagnosis, conventional chromosome analysis may also be more informative.
Reference Values
Not applicable
Interpretation
If notified by the client, this test may be canceled, and a processing fee will be assessed.
If no notification to proceed with testing is received, this test will be reported as "cancelled."
Cautions
No significant cautionary statements
Method Description
The specimen will be held in the laboratory while preliminary morphologic assessment is in process to determine if fluorescence in situ hybridization (FISH) testing is appropriate.
Day(s) Performed
Monday through Sunday
Report Available
4 daysSpecimen Retention Time
4 weeksPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
See individual reflex tests
LOINC Code Information
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 51836 | Result Summary | 50397-9 |
| 51838 | Interpretation | 69965-2 |
| CG666 | Reason for Referral | 42349-1 |
| CG667 | Specimen | 31208-2 |
| 51839 | Source | 31208-2 |
| CG791 | Requested FISH Test | 48767-8 |
| 51841 | Method | 85069-3 |
| 53433 | Additional Information | 48767-8 |
| 51842 | Released by | 18771-6 |