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HelpMayo Test ID HPWET Hematopathology Consultation, MCL Embed
Ordering Guidance
1. If requesting a peripheral blood smear evaluation only, order SPSM / Morphology Evaluation (Special Smear), Blood.
2. If requesting a hematopathology consultation on paraffin-embedded tissue and slides, order PATHC / Pathology Consultation. Also include a cover letter indicating hematopathology review requested.
3. If requesting a hematopathology consultation and only paraffin-embedded biopsy/clot samples and bone marrow aspirate are submitted, order HPCUT / Hematopathology Consultation, Client Embed. Orders for HPCUT require MCL prior authorization.
Additional Testing Requirements
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) to the outside of the transport container before putting into the courier mailer.
Necessary Information
All requisition and supporting information must be submitted in English.
Each of the following items is required:
1. All requisitions must be labeled with:
-Patient name, date of birth, medical record number, and either case number or pathology ID
-Name and phone number of the referring pathologist or ordering provider
-Collection date
2. Hematopathology Patient Information (T676); print and submit with the case
3. A recent Hematology/Oncology clinical note; print and submit with the case
4. Complete blood cell count (CBC) results from testing performed within 14 days of bone marrow collection.
5. Indicate clinical reason/context
6. All pending and final reports for ancillary testing on submitted specimens.
Specimen Required
All specimens are required to perform testing.
Supplies: Bone Marrow Collection Kit (T793)
Additional Information:
All specimens and paperwork must be labeled with:
-Two patient identifiers (patient name, date of birth, medical record number, case number, or pathology ID)
-Specimen type
Information on collecting, packaging, and shipping specimens is available:
-Bone Marrow Core Biopsy, Clot, and Aspirate Collection Guideline
-Assistance with Bone Marrow Collection
Specimen Type: Bone marrow aspirate
Slides: 5
Preferred: Freshly prepared slides made at the time of specimen collection
Acceptable: Slides made from EDTA bone marrow specimen, within 2 hours of collection
Submission Container/Tube: Plastic slide holder
Specimen Volume: 5 Total slides: 2 direct smears and 3 unit prep slides; unfixed and unstained, per unilateral collection
Collection Instructions:
1. Prepare slides of bone marrow aspirate.
2. If bone marrow units are sparse or absent or aspirate is a dry tap, make biopsy touch prep slides.
3. Air dry slides. Do not place on hot plate to dry.
4. Place Parafilm around the slide carriers holding unstained slides to prevent exposure to formalin fumes during transport. Place slides in a separate bag apart from any formalin-fixed clot or core biopsy specimens. If using slide carriers, make sure they have not been used to carry fixed slides previously.
Specimen Type: Bone marrow aspirate
Container/Tube: Lavender top (EDTA) and yellow top (ACD solution A or B)
Specimen Volume: 2 x 3 mL in EDTA and 2 x 6 mL in ACD solution A or B
Collection Instructions:
1. Aspirate per standard bone marrow collection procedure.
2. Send bone marrow specimens in original tubes. Do not aliquot.
Specimen Type: Bone marrow aspirate clot
Container/Tube: Bone marrow aspirate clot in 10% formalin
Specimen Volume: 0.5 mL
Collection Instructions:
1. Place 0.5 mL bone marrow aspirate in clot tube.
2. After clot has formed, place clot in 10% formalin.
3. Place Parafilm around the container to prevent exposure.
Specimen Type: Bone marrow core biopsy
Container/Tube: Fixed biopsy core in 10% formalin
Collection Instructions:
1. If bone marrow units are sparse or absent, or aspirate is a dry tap, make biopsy touch prep slides.
2. Place biopsy core in 10% formalin immediately after collection.
3. Fix in 10% formalin for 1 to 2 hours.
4. Place Parafilm around the 10% formalin container to prevent exposure.
Specimen Type: Blood
Slides: 2
Preferred: 2 Freshly prepared fingerstick slides
Acceptable: 2 Slides made from whole blood in EDTA, within 8 hours of collection
Submission Container/Tube: Plastic slide holder
Specimen Volume: 2 Unstained and unfixed slides
Collection Instructions:
1. Prepare 2 smears of even thickness.
2. Place Parafilm around the slide carriers holding unstained slides to prevent exposure to formalin fumes during transport. Place slides in a separate bag apart from any formalin-fixed clot or core biopsy specimens. If using slide carriers, make sure they have not been used to carry fixed slides previously.
Useful For
Obtaining a rapid, expert opinion for diagnosis of hematologic and non-hematologic diseases using unprocessed bone marrow biopsy specimens referred by the primary pathologist
Obtaining special studies that are not available locally
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| BMAPC | Bone Marrow Aspirate | No, (Bill Only) | No |
| BMBPC | Bone Marrow Biopsy | No, (Bill Only) | No |
| BMCPC | Bone Marrow Clot | No, (Bill Only) | No |
| DCALP | Decalcification | No, (Bill Only) | No |
| PBPC | Peripheral Blood | No, (Bill Only) | No |
| PBTC | Peripheral Blood, TC | No, (Bill Only) | No |
| PPPC | Particle Prep | No, (Bill Only) | No |
Testing Algorithm
Laboratory approval is required prior to ordering this test. Contact Mayo Clinic Laboratories at 800-533-1710.
A Mayo Clinic hematopathologist will provide a full bone marrow workup that includes an evaluation of the specimen and determination of a diagnosis provided within a formal pathology report.
Ancillary Testing:
Based on Mayo Clinic-approved algorithms or at a staff hematopathologist's discretion, ancillary testing may be performed to assist in rendering an accurate diagnosis and provide important prognostic information. These test results (eg, cytochemical stains on bone marrow aspirate smear, immunohistochemical stains on bone marrow biopsy or clot sections, chromosome analysis, fluorescence in situ hybridization, flow cytometry, microarray, molecular and/or next-generation sequencing testing) will be reported and billed separately. While reported separately, these results will continue to be considered and referred to in the final pathology interpretation.
If ancillary testing (eg, flow cytometry) is desired by the client outside of this consultation, each test must be ordered separately. Tests ordered outside of the consultation may or may not be integrated into the final pathology report based on the staff hematopathologist's discretion.
If the volume of bone marrow aspirate is limited, prioritization of testing will be determined by the staff hematopathologist. Testing requested or suggested by the referring physician (immunostains, molecular studies, etc) may not be performed if deemed unnecessary by the reviewing staff hematopathologist.
Note: Calls are not routinely made; however, depending on the nature of the case, a call may be placed to the ordering provider or pathologist. These situations include, but are not limited to, a new diagnosis of acute leukemia or aggressive high-grade lymphoma. To contact a Mayo Clinic hematopathologist, call the Hematopathology Communications team at 507-284-5600.
For more information see:
Special Instructions
Reporting Name
Hematopathology ConsultSpecimen Type
VariesSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient | ||
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Clinical Information
Diagnosis of a hematologic disease requires thorough and accurate morphologic examination of peripheral blood and bone marrow as well as interpretation of ancillary testing results (eg, cytochemistry, immunohistochemistry, flow cytometric immunophenotyping, chromosome analysis, and fluorescence in situ hybridization and molecular testing) by a highly qualified hematopathologist. With recent advent of new understanding and treatment options, more ancillary tests are available. Efficient utilization and accurate interpretation of these tests are crucial for patient care. These tests can assist in rendering an accurate diagnosis and could provide prognostic prediction and potential indication or guidance of therapy.
Reference Values
An interpretive report will be provided.
Interpretation
Results of the consultation are reported in a formal pathology report that includes a description of ancillary test results (if applicable) and an interpretive comment. When the case is completed, results may be communicated by a phone call.
Cautions
All appropriate stained/unstained slides, biopsy tissue, and aspirate are required to make a diagnosis. The referring pathologist's and clinician's name and phone numbers are essential. Specific diagnosis may require correlation with clinical information; this information must be sent with the specimen.
Method Description
All requests will be processed as a consultation case. Ancillary testing will be performed as appropriate to be diagnostically indicated and at an additional charge.
Day(s) Performed
Monday through Saturday
Report Available
3 to 7 days; Cases requiring additional material or ancillary testing may require additional time.Specimen Retention Time
Specimens embedded and slides prepared at Mayo Clinic: Indefinitely; Bone marrow aspirate: 2 weeksPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
85007 (if appropriate)
85060 (if appropriate)
85097 (if appropriate)
88305 (if appropriate)
88311 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HPWET | Hematopathology Consult | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 71098 | Interpretation | 60570-9 |
| 71099 | Participated in the Interpretation | No LOINC Needed |
| 71100 | Report electronically signed by | 19139-5 |
| 71101 | Addendum | 35265-8 |
| 71102 | Gross Description | 22634-0 |
| 71446 | Material Received | 85298-8 |
| 71103 | Disclaimer | 62364-5 |
| 71827 | Case Number | 80398-1 |
Test Classification
Not ApplicableMethod Name
Medical Interpretation
Specimen Minimum Volume
See Specimen Required
Clinical Reference
Sundaram S, Jizzini M, Lamonica D, et al. Utility of bone marrow aspirate and biopsy in staging of patients with T-cell lymphoma in the PET-Era-tissue remains the issue. Leuk Lymphoma. 2020;61(13)3226-3233. doi:10.1080/10428194.2020.1798950