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HelpMayo Test ID JCV JC Virus Detection by In Situ Hybridization
Shipping Instructions
Attach the green pathology address label included in the kit to the outside of the transport container.
Necessary Information
A pathology/diagnostic report and a brief history are required.
Specimen Required
Specimen Type: Formalin-fixed, paraffin-embedded tissue block
Supplies: Pathology Packaging Kit (T554)
Specimen Volume: Entire block
Specimen Type: Slides
Slides: 4 Unstained glass, positively-charged slides with 5 (+ or - 1)-microns formalin-fixed, paraffin-embedded tissue
Additional Information: Broken or cracked unstained slides will be rejected. Stained slides that were broken and repaired (ie, double mounted) at the client site will not be rejected, as, in most cases, they can still be reviewed under the microscope. However, due to the double mounting, digital scanning cannot be performed.
Useful For
Confirming a clinical and histopathologic diagnosis of progressive multifocal leukoencephalopathy
Method Name
In Situ Hybridization (ISH)
Reporting Name
JC Virus ISHSpecimen Type
SpecialSpecimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Special | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
| Wet/frozen tissue | Reject |
| Cytology smears | Reject |
| Non-formalin fixed tissue | Reject |
| Nonparaffin embedded tissue | Reject |
| Noncharged, ProbeOn or Snowcoat slides | Reject |
| Broken or cracked unstained slides | Reject |
| Broken or cracked stained slides that cannot be repaired | Reject |
Clinical Information
JC virus is the etiologic agent of progressive multifocal leukoencephalopathy (PML), a rare, demyelinating, fatal disorder of the central nervous system that occurs on a background of immune deficiency. PML is an infrequent complication of a wide variety of conditions, including lymphoproliferative disorders (Hodgkin disease, chronic lymphocytic leukemia), sarcoidosis, tuberculosis, and AIDS.
Interpretation
This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.
Cautions
Studies have shown potential cross reactivity between polyoma (BK) and JC virus in situ hybridization (ISH) testing.(1)
Age of a cut paraffin section can affect staining quality. Stability thresholds vary widely among published literature. Best practice is for paraffin sections to be cut within 6 weeks.
Broken or cracked unstained slides are not compatible with the instrumentation used in our performing laboratories and will be rejected. Additional material may be requested.
Attempts will be made to repair broken or cracked stained slides; however, if they are unable to be repaired (ie, shattered in transit), they will be rejected and discarded due to safety concerns.
Clinical Reference
1. Fritzsche FR, Pianca S, Gaspert A, et al: Silver-enhanced in situ hybridization for detection of polyomavirus DNA in patients with BK virus nephropathy. Diagn Mol Pathol. 2011 Jun;20(2):105-110
2. Aksamit AJ, Mourrain P, Sever JL, Major EO: Progressive multifocal leukoencephalopathy: investigation of 3 cases using in situ hybridization with JC virus biotinylated DNA probe. Ann Neurol. 1985 Oct;18(4):490-496
3. Aksamit AJ: Nonradioactive in situ hybridization in progressive multifocal leukoencephalopathy. Mayo Clin Proc. 1993 Sept;68(9):899-910
4. Muaoz-Marmol AM, Mola G, Fernandez-Vasalo A, et al: JC virus early protein detection by immunohistochemistry in progressive multifocal leukoencephalopathy: a comparative study with in situ hybridization and polymerase chain reaction. J Neuropathol Exp Neurol. 2004 Nov;63(11):1124-1130
5. Vago L, Cinque P, Sala E, et al: JCV-DNA and BKV-DNA in the CNS tissue and CSF of AIDS patients and normal subjects. Study of 41 cases and review of the literature. J Acquir Immune Defic Syndr Hum Retrovirol. 1996 Jun 1;12(2):139-146
6. Chen H, Chen XZ, Waterboer T, et al: Viral infections and colorectal cancer: a systematic review of epidemiological studies. Int J Cancer. 2015 Jul 1:137(1)12-24
Method Description
In situ hybridization on sections of paraffin-embedded tissue.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Report Available
5 to 7 daysSpecimen Retention Time
Until staining is complete.Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88365-Primary
88364-If additional ISH
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| JCV | JC Virus ISH | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 71214 | Interpretation | 50595-8 |
| 71215 | Participated in the Interpretation | No LOINC Needed |
| 71216 | Report electronically signed by | 19139-5 |
| 71218 | Material Received | 81178-6 |
| 71596 | Disclaimer | 62364-5 |
| 72115 | Case Number | 80398-1 |