Home
HelpMayo Test ID LAGGT Granulocyte Antibodies, Serum
Specimen Required
Collection Container/Tube: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Additional Information: Only pretransfusion reaction specimen is acceptable.
Useful For
Work-up of individuals having febrile, nonhemolytic transfusion reactions
Detection of individuals with autoimmune neutropenia
This test is not useful for the diagnosis of neutropenia caused by marrow suppression by drugs or tumors.
Method Name
Indirect Immunofluorescence
Reporting Name
Granulocyte Ab, SSpecimen Type
Serum RedSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum Red | Refrigerated (preferred) | 30 days | |
| Frozen | 365 days | ||
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Clinical Information
Granulocyte antibodies are induced by pregnancy or prior transfusion and are associated with febrile, nonhemolytic transfusion reactions. Patients who have been immunized by previous transfusions, pregnancies, or allografts frequently experience febrile, nonhemolytic transfusion reactions that must be distinguished from hemolysis before further transfusions can be safely administered. Granulocyte antibodies may also be present in autoimmune neutropenia.
Reference Values
Not applicable
Interpretation
A positive result in an individual being worked up for a febrile transfusion reaction indicates the need for leukocyte-poor (filtered) red blood cells.
This test cannot distinguish between allo- and autoantibodies
Cautions
No significant cautionary statements
Clinical Reference
1. Flesch BK, Reil A. Molecular genetics of the human neutrophil antigens. Transfus Med Hemother. 2018;45(5):300-309. doi:10.1159/000491031
2. Gottschall JL, Triulzi DJ, Curtis B, et al. The frequency and specificity of human neutrophil antigen antibodies in a blood donor population. Transfusion. 2011;51(4):820-827. doi:10.1111/j.1537-2995.2010.02913.x
Method Description
Purified granulocyte preparations from normal donors are incubated with patient's test serum and then with fluorescein-tagged antihuman globulin reagent. Sera containing the antibodies deposit immunoglobulin on the target cell membrane, which is detected by the second stage antibody and visualized by fluorescence microscopy.(Verheugt FW, von dem Borne AE, Decary F, Engelfreit CP. The detection of granulocyte alloantibodies with an indirect immunofluorescence test. Br J Haematol. 1997;36(4):533-534)
Day(s) Performed
Tuesday, Wednesday, Friday
Report Available
7 to 15 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
86021
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LAGGT | Granulocyte Ab, S | 35279-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| LAGG | Granulocyte Ab, S | 35279-9 |