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HelpMayo Test ID LCADP Adenovirus, Molecular Detection, PCR, Plasma
Specimen Required
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Screw-capped, sterile container
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot plasma into a sterile, plastic vial.
Useful For
Aiding in diagnosing adenovirus infections using plasma specimens
Method Name
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Reporting Name
Adenovirus PCR, PSpecimen Type
Plasma EDTASpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reject Due To
| Gross hemolysis | Reject |
Clinical Information
Human adenoviruses cause a variety of diseases, including pneumonia, cystitis, conjunctivitis, diarrhea, hepatitis, myocarditis, and encephalitis. In humans, adenoviruses have been recovered from almost every organ system. Infections can occur at any time of the year and in all age groups. Currently, there are over 50 adenovirus serotypes that have been grouped into 6 separate subgenera.
Although adenovirus can be recovered in cell culture, it can take up to 3 weeks for the virus to be identified by culture methods (Mayo's shell vial culture provides more rapid results, reported at 2 and 5 days). Polymerase chain reaction assays offer a rapid, specific, and sensitive means of diagnosis by detecting adenovirus DNA.
Reference Values
Negative
Interpretation
A positive result indicates the presence of adenovirus nucleic acid in the clinical specimen.
A negative result does not rule out the presence of adenoviruses because viral DNA may be present at levels below the detection limits of this assay.
Cautions
Test results should be used as an aid in diagnosis and should not be considered diagnostic in themselves.
Although the reference range is generally considered to be "Negative" for this assay, adenovirus DNA may be detected from asymptomatic individuals in certain settings. This assay should only be used to test patients with clinical history and symptoms consistent with adenovirus disease, and is not used to screen healthy patients.
Clinical Reference
1. Florescu DF, Schaenman JM: Adenovirus in solid organ transplant recipients: Guidelines from the American Society of Transplantation Infectious Diseases Community of Practice. Clin Transplant. 2019 Sep;33(9):e13527
2. Buckwalter SP, Teo R, Espy MJ, Sloan LM, Smith TF, Pritt BS: Real-time qualitative PCR for 57 human adenovirus types from multiple specimen sources. J Clin Microbiol. 2012 Mar;50(3):766-771. doi:10.1128/jcm.05629-11
3. Ebner K, Pinsker W, Lion T: Comparative sequence analysis of the hexon gene in the entire spectrum of human adenovirus serotypes: phylogenetic, taxonomic, and clinical implications. J Virol. 2005 Oct;79(20):12635-12642
4. Ebner K, Suda M, Watzinger F, Lion T: Molecular detection and quantitative analysis of the entire spectrum of human adenoviruses by a two-reaction real-time PCR assay. J Clin Microbiol. 2005 Jul;43(7):3049-3053
5. Jothikumar N, Cromeans TL, Hill VR, Lu X, Sobsey MD, Erdman DD: Quantitative real-time PCR assays for the detection of human adenoviruses and identification of serotypes 40 and 41. Appl Environ Microbiol. 2005 Jun;71(6):3131-3136
6. Robinson C, Echavarria M: Adenovirus. In: Murray PR, Baron EJ, Jorgensen JH, eds. Manual of Clinical Microbiology. ASM Press; 2007:1589-1600
7. Kaneko H, Maruko I, Iida T, et al: The possibility of human adenovirus detection from the conjunctiva in asymptomatic cases during a nosocomial infection. Cornea 2008 Jun;27(5):527-530
Method Description
Respiratory, swabs, stools, tissues, plasma, and urine samples are processed according to specimen source. Viral nucleic acid is extracted by the MagNA Pure automated instrument (Roche Applied Science). Primers and fluorescence resonance energy transfer (FRET) probes target a relatively conserved 185 base-pair region of the adenovirus penton gene. The LightCycler instrument (Roche Applied Science) amplifies and monitors the development of target nucleic acid sequences after the annealing step during polymerase chain reaction (PCR) cycling. This automated PCR system rapidly detects amplicon development through stringent air-controlled temperature cycling in capillary cuvettes. The detection of amplified products is based on the FRET principle. For FRET product detection, a hybridization probe with a donor fluorophore, fluorescein, on the 3'-end is excited by an external light source and emits light that is absorbed by a second hybridization probe with an acceptor fluorophore, LC-Red 640, at the 5'-end. The acceptor fluorophore then emits a light of a different wavelength that can be measured with a signal that is proportional to the amount of specific PCR product.(Unpublished Mayo method)
Day(s) Performed
Monday, Wednesday, Friday
Report Available
2 to 5 daysSpecimen Retention Time
1 weekPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LCADP | Adenovirus PCR, P | 21055-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 56088 | Adenovirus PCR, P | 21055-9 |