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HelpMayo Test ID MYGLS Myoglobin, Serum
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Useful For
Assessing muscle damage from any cause
Method Name
Electrochemiluminescent Immunoassay (ECLIA)
Reporting Name
Myoglobin, SSpecimen Type
SerumSpecimen Minimum Volume
0.6 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 365 days | ||
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Clinical Information
Myoglobin is a heme protein found in smooth and skeletal muscles. Serum myoglobin reflects a balance between intravascular release of myoglobin from muscle and renal clearance.
Previously serum myoglobin had been advocated as a sensitive marker for early acute myocardial injury (eg, acute myocardial infarction: AMI). However, more recent studies indicate that newer markers (eg, troponin) provide superior diagnostic utility in detecting early myocardial injury.
Elevation of serum myoglobin may occur as a result of muscle trauma, resuscitation, myopathies, AMI, shock, strenuous body activity, or decreased elimination during renal insufficiency. Extreme elevations occur in rhabdomyolysis. Creatine kinase is released from muscle and used more commonly for this purpose.
Reference Values
Males: 0 to 72 mcg/L
Females: 0 to 58 mcg/L
Interpretation
Elevated myoglobin levels are seen in conditions of acute muscle injury.
Cautions
Elevation is nonspecific for acute myocardial infarction. The test is of no value in this regard in the presence of kidney failure, rhabdomyolysis, extensive trauma, acute peripheral vascular occlusion, or after seizures.
Serum levels rise in renal insufficiency.
In very rare cases, gammopathy, in particular type IgM (Waldenstrom macroglobulinemia), may cause unreliable results.
Clinical Reference
1. Lamb EJ, Jones GRD. Kidney functions tests. In: Rifai N, Horvath AR, Wittwer CT, eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:489
2. Cappenllini MD, Lo SF, Swinkels DW. Hemoglobin, iron, bilirubin. In: Rifai N, Horvath AR, Wittwer CT, eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:747
Method Description
This myoglobin test is a sandwich-principal assay. The first incubation is 9 mcL of sample, a biotinylated monoclonal myoglobin-specific antibody, and a monoclonal myoglobin-specific antibody labeled with a ruthenium complex, which react to form a sandwich complex. In the second incubation, the complex becomes bound to the solid phase via interaction of biotin and streptavidin after addition of streptavidin-coated microparticles. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Results are determined via a calibration curve, which is instrument specifically generated by 2-point calibration, and a master curve provided via the cobas link.(Package insert: Elecsys Myoglobin. Roche Diagnostics; 10/2022)
Day(s) Performed
Monday through Sunday
Report Available
1 to 2 daysSpecimen Retention Time
7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
83874
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MYGLS | Myoglobin, S | 2639-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MYGLS | Myoglobin, S | 2639-3 |