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HelpMayo Test ID PPAP Parental Sample Prep for Prenatal Microarray Testing, Blood
Additional Testing Requirements
This test must be ordered in conjunction with CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue. Each parent should have a specimen collected for this test ID using a different order number than the fetal specimen collected for the CMAP, CMAPC, or CMAMT order.
Necessary Information
1. The reason for testing is required.
2. Include the name listed on the prenatal specimen that was submitted for CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue.
Specimen Required
This test requires 2 blood specimens: 1 sodium heparin and 1 EDTA
Specimen Type: Whole blood
Container/Tube: Green top (sodium heparin) and lavender top (EDTA)
Specimen Volume: 3 mL EDTA and 4 mL sodium heparin
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimens in original tubes. Do not aliquot.
Useful For
Preparing parental blood specimen for possible confirmation testing if an abnormality is detected on the prenatal array sample
DNA extraction of the maternal blood specimen used for maternal cell contamination testing
Testing Algorithm
This test contains no charge and serves as a way to correlate proband and parental specimens. If additional testing is warranted, the appropriate tests will be added.
When CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue is ordered, parental blood specimens are collected and prepared for confirmation studies should an abnormality be detected on the prenatal sample. If an abnormality is detected on the fetal specimen, the most appropriate testing (microarray, FISH, or chromosome analysis) to aid in the interpretation of the prenatal result can be performed on the parental specimens. A paternal blood specimen is requested but not required.
Maternal cell contamination testing will be performed on the maternal blood and prenatal specimens to rule out the presence of maternal cells in the fetal sample.
Reporting Name
Parental Prenatal Array Prep TestSpecimen Type
Whole bloodSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole blood | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Clinical Information
In order to interpret equivocal array results on a prenatal sample (amniotic fluid or chorionic villus), parental studies are performed to determine if the abnormality detected on the prenatal array is inherited or de novo.
Maternal cell contamination testing is performed on the maternal blood and prenatal specimen to detect the presence of maternal cells in the fetal sample.
Reference Values
An interpretive report will be provided.
Interpretation
No interpretation will be provided. This test is for specimen processing only.
Cautions
No significant cautionary statements
Method Description
The heparinized sample will be cultured following standard laboratory protocols and a fixed-cell pellet will be prepared. DNA will be extracted from the EDTA sample and used to perform maternal cell contamination testing on the maternal sample. The fixed cell pellet and DNA will be stored in the laboratory for possible confirmation testing if an abnormality is detected on the prenatal array sample.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Report Available
VariesSpecimen Retention Time
4 weeksPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
This test ID contains no charge and serves as a way to correlate proband parental specimens. If additional testing is warranted, the appropriate tests will be added.
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PPAP | Parental Prenatal Array Prep Test | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 55079 | Result Summary | 50397-9 |
| 52978 | Interpretation | 69965-2 |
| 52979 | Reason For Referral | 42349-1 |
| 53337 | Specimen | 31208-2 |
| 53338 | Source | 31208-2 |
| 53339 | Method | 85069-3 |
Specimen Minimum Volume
2 mL EDTA and 2 mL sodium heparin whole blood