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Mayo Test ID SUBIF Immunoglobulin G (IgG) Subtypes Immunofluorescence, Tissue


Ordering Guidance


If additional interpretation/analysis is needed, request PATHC / Pathology Consultation along with this test and send the corresponding renal pathology light microscopy and immunofluorescence (IF) slides (or IF images on a CD), electron microscopy images (prints or CD), and the pathology report.



Shipping Instructions


1. Advise shipping specimens in Styrofoam transportation coolers to avoid extreme hot or cold temperatures to ensure specimens are received at required specimen stability temperature.

2. Attach the green pathology address label included in the kit to the outside of the transport container.



Necessary Information


A pathology/diagnostic report is required.



Specimen Required


Preferred: Frozen tissue

Supplies: Renal Biopsy Kit (T231)

Specimen Type: Kidney tissue

Container/Tube: Renal Biopsy Kit, Zeus/Michel's, Frozen

Specimen Volume: Entire specimen

Collection Instructions: Collect specimens according to the instructions in Renal Biopsy Procedure for Handling Tissue for Light Microscopy, Immunofluorescent Histology, and Electron Microscopy.

Additional Information: If standard immunoglobulin and complement immunofluorescence has already been performed, submit the residual frozen tissue (must contain glomeruli) on dry ice.

 

Acceptable: Frozen tissue

Slides: 4 frozen tissue unstained positively-charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.


Useful For

Determining the subclass of IgG antibody found in renal immunofluorescent panel and determining if the deposits are monoclonal or monotypic

Method Name

Direct Immunofluorescence

Reporting Name

IGG Subtypes Immunofluorescence

Specimen Type

Special

Specimen Stability Information

Specimen Type Temperature Time Special Container
Special Frozen (preferred)
  Ambient 
  Refrigerated 

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Clinical Information

IgG subtypes are helpful in confirming some disease processes affecting the kidney.

Cautions

No significant cautionary statements

Clinical Reference

Hemminger J, Nadasdy G, Satoskar A, Brodsky SV, Nadasdy T: IgG subclass staining in routine renal biopsy material. Am J Surg Pathol. 2016 May;40(5):617-626

Method Description

Direct immunofluorescence staining on sections of fresh or frozen tissue.(Unpublished Mayo method)

Day(s) Performed

Monday through Friday

Report Available

1 to 2 days

Specimen Retention Time

5 years

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88346-primary IF

88350-if additional IF

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SUBIF IGG Subtypes Immunofluorescence In Process

 

Result ID Test Result Name Result LOINC Value
BA0271 Interpretation 50595-8
BA0272 Participated in the Interpretation No LOINC Needed
BA0275 Report electronically signed by 19139-5
BA0276 Addendum 35265-8
BA0274 Gross Description 22634-0
BA0273 Material Received 22633-2
71618 Disclaimer 62364-5
71850 Case Number 80398-1

Interpretation

Staining intensity is graded as negative (0), weak (trace, 1+), moderate (2+) and strong (3+) and will be reported as such when not accompanied by a pathology consultation request.